unkown microbe from patient sample lab report

The unknown #123 contained two different specimen of bacteria, one being a gram positive bacteria and one being a gram negative bacteria. The first unknown in #123 found to be a gram negative bacteria, was identified as Pseudomonas aeruginosa. This identification was reached by only one biochemical test and close observation.

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What is unknown microbiology lab report?

Unknown Microbiology Lab Report Essay. UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics.

How do you find the unknowns in a bacterial culture?

The first step to figuring out the unknowns, was to separate the two bacteria. In order to do this, a nutrient agar plate was used. The streak method was used to spread the bacteria across the nutrient agar in hopes of isolating a pure culture of one of the bacteria.

What is an example of a lab report in microbiology?

Example of Unknown Lab Report, Microbiology. The quadrant streak helped spread out the bacteria and made it easier for the two different bacteria to make colonies that could be differentiated by the naked eye. This nutrient agar was incubated at 37° C for two days. The results were checked to see if two separate bacteria were growing.

What is the name of the unknown bacteria in this experiment?

The Unknown bacteria is Enterobacter aerogenes The other cultured bacteria was gram stain using same procedure at Number 4 The second streak culture was subjected to microscopic test. In a 100x magnification the color of bacteria was PURPLE and the shape is ROUND, GRAPE LIKE STRUCTURE.

How do you write an unknown bacteria for a lab report?

For example, instead of writing "I used a TSA agar plate to isolate my unknown," it is customary to write, "A trypticase soy agar (TSA) plate was used to isolate the unknown." It is also customary to write in the past tense for most of the report.

How can an unknown sample of bacteria be identified?

Identifying the Bacterium If you have an unknown bacteria and you want to identify it, you'll typically perform a gram stain and then observe the colony appearance and the individual features. At that point, you can say you have, for example, a gram-negative, aerobic streptobacilli.

How do you write a lab report for microbiology?

A lab report differs from a paper in that it has defined sections. The sections required vary from laboratory to laboratory but the standard outline for most lab reports in the biological science include: title, your name, purpose of the experiment, methods, results, discussion and conclusion, references.

How do you create an unknown project in microbiology?

0:0431:58Unknown Project Beginning - YouTubeYouTubeStart of suggested clipEnd of suggested clipSo just to give you an idea of how the unknown project would work is that you would be given a brothMoreSo just to give you an idea of how the unknown project would work is that you would be given a broth. And in the broth that would contain two types of bacteria. You would have one gram positive.

What are the 3 main approaches to the identification of unknown bacteria?

What three main approaches can be used by microbiologists to identify microorganisms? -Phenotypic- observing bacterial morphology and staining properties as well as biochemical testing. You just studied 45 terms!

How do you identify a microbe?

PCR, including Real-Time PCR, is probably the most widely used molecular technique for identifying microbes. Using PCR, one can rapidly detect and identify microbial species directly from clinical samples, thus speeding up diagnostic procedures.

Why is identifying an unknown bacteria important?

WHY IS THE IDENTIFICATION OF BACTERIAL UNKNOWNS IMPORTANT? Microbiologists must identify bacterial isolates for several practical reasons: • Medical diagnostics — identifying a pathogen isolated from a patient. Food industry — identifying a microbial contaminant responsible for food spoilage.

How do you write a lab report?

A lab report is broken down into eight sections: title, abstract, introduction, methods and materials, results, discussion, conclusion, and references. The title of the lab report should be descriptive of the experiment and reflect what the experiment analyzed.

What is the purpose of the unknown project in microbiology?

0:2454:29Unknown Project Instructions - YouTubeYouTubeStart of suggested clipEnd of suggested clipAnd so the purpose of the unknown. Project is to identify unknown bacteria.MoreAnd so the purpose of the unknown. Project is to identify unknown bacteria.

What is the purpose of unknown lab report?

The goal of this lab report is 1) to demonstrate comprehension of the methods and lab techniques learned during the semester 2) to explain the tests performed on each isolated unknown that led to the identification of each unknown 3) and to give a background on the characteristics, pathogenicity and some uses of one of ...

What are the common biochemical tests used in the identification of bacteria?

Summary of Biochemical TestsCatalase Test.Mannitol Salt Agar (MSA)Blood Agar Plates (BAP) Streak-stab technique.Taxos P (optochin sensitivity testing)Taxos A (bacitracin sensitivity testing)CAMP Test.Bile Esculin Agar.Nitrate Broth.More items...

What was the unknown bacteria?

After a series of differential tests, a chart of bacteria was referenced to discover the unknown bacteria was E. coli. The Gram Stain resulted in a Gram-negative rod shaped bacteria which was not colonized.

Why is it important to know the identity of microorganisms?

Introduction. It is important to know the identity of microorganisms for knowing how bacteria works and how it is structured means knowing how it can affect humans. By knowing which agents are causing a disease, one can determine the correct treatment necessary with persons afflicted with the microbes in question.

How long does it take for E. coli to clear?

The body generally clears the bacterial infection on its own within 7-10 days. Unknown Lab Report: Unknown “C”.

Can E. coli make you sick?

According to the Center of Disease Control (CDC), “Although most strains of E. coli are harmless, others can make you sick. Some kinds of E. coli can cause diarrhea, while others cause urinary tract infections, respiratory illness and pneumonia, and other illnesses” (CDC).

What bacteria turned out to be Gram negative?

For further accurate results inoculated plate was kept in the incubator for another 48 hours. The result was confirmed to be Gram negative bacillus.

Why is knowing the differences in bacterial structure and response important?

Knowing the differences in bacterial structure and response is essential for all future and current healthcare providers. Dealing with people’s lives and health is it necessary to know what kind of antibiotic can be prescribed to a patient in order to fix his problem.

What is the Gram positive bacteria?

According to microbiology instructor Gram positive bacteria was Staphylococcus aureus, that leads to the conclusion that final Urea test had shown wrong results. For future healthcare providers, it is extremely important to be able to identify what kind of pathogen is thriving in a patient’s organism.

Why did the nitrate and MR-VP test turn out to be negative?

Nitrate and MR-VP tests were supposed to be redone, due to a technical mistake. After accurately conducted test, the results of Nitrate test and MR-VP tests turned out to be negative. Indole test was negative as well and didn’t show any color change.

Why do different microorganisms require different environments?

Different microorganisms require various environments in order to replicate and to become dangerous for a person’s health. An integral part of any medical treatment is to be able to recognize and identify a specific bacterium that can create potentially a big problem for a patient.

Why are microorganisms important?

Microorganisms cause a great amount of diseases. For healthcare providers it is very important to be aware of what organisms are pathogenic and cause a disease and, therefore, to find an appropriate treatment . Different microorganisms require various environments in order to replicate and to become dangerous for a person’s health.

Can staph be spread through physical contact?

Under normal circumstances it doesn’t cause any problems , however, if a patient has a compromised immune system Staphylococcus aureus can become a serious problem and lead to Methicillin resistant Staphylococcus aureus (MRSA). MRSA is a type of staph bacteria that can be easily spread through a physical contact with a sick person.

What is the purpose of the microbiology lab?

The purpose of this laboratory exercise is to evaluate the student’s knowledge and capabilities in identifying micro organism. Knowing what organism will give the student an idea of the genus and species that can cause diseases, how it can grow and reproduce including how this type of bacteria can be treated and killed, what type of antibiotic it will be susceptible to.

What is the gram positive bacteria?

The unknown gram positive bacteria is Staphylococcus aureus. Some problems encountered on this activity is the handling and contamination.

What is the purpose of a sterilized inoculating loop?

Using a sterilized inoculating loop a drop distilled water in a dry glass slide which was previously soaked in alcohol. The purpose of the water is to make a pool so that the Unknown specimen can be transferred and spread in the slide.

What enzymes are used to detect hydrogen peroxide?

The bacteria detect enzymes amylase. Amylase is the enzymes that chopped up the Hydrogen Peroxide to water and Oxygen .NitratesTo determine the ability of bacteria to convert NO3 to NO2Nitrate broth, Reagent A , Reagent BThere’s no change in color.

What test was used to test for Gram Negative Unknown?

Maltose. Oxidase. An MSA and EMB test were performed using the Unknown 109 to try to promote the gram negative unknown to grow, but it did not work. The professor gave an Alternate #4 tube, so the gram negative unknown could be found.

What is the correct identification for gram positive bacteria?

The correct identification for the gram positive bacterium was Bacillus cereus. The B. cereus was found using the solution in the Unknown 109 test tube. Originally, a sample from the Unknown tube was supposed to be inoculated on a nutrient agar plate using the quadrant streak technique. This was to allow the gram positive and gram negative to be spread out, creating separate colonies. This never occurred. Several nutrient agar plates where inoculated and two different bacteria never grew. Then, it was concluded that only one bacterium was growing on the nutrient agar, so a gram stain was performed. A gram positive rod was the outcome, ruling out the gram positive cocci. The Methyl Red test concluded positive for the presence of glucose fermenters. The Casein test concluded negative for the bacterium having casease to hydrolyze casein. This was an incorrect finding because B. cereus does have casease. The finding could have been incorrect because the clearing around the bacterium might not have been as clear for the tester to notice. The Glycerol and Maltose test showed fermentation for carbohydrates by changing the solution from yellow to red. The Maltose test showed positive, but the Glycerol test show incorrect results for negative. The two tests call for the bacterium to be in a liquid form when added to the test tubes, but the test was done from the bacterium being on an agar jell. The bacterium might not have mixed well with the Glycerol solution, creating an improper answer. Lastly, an Oxidase test showed positive for the bacterium having cytochrome c. This completed the search for the Gram positive rod: B. cereus (3).

Why is the casein test negative?

The Casein test concluded negative for the bacterium having casease to hydrolyze casein. This was an incorrect finding because B. cereus does have casease. The finding could have been incorrect because the clearing around the bacterium might not have been as clear for the tester to notice.

What is the significance of the EMB test?

They were positive and acidic. The EMB test produced green metallic colonies, which was a big flag that the bacterium might be Escherichia coli. The Methyl Red test was positive for the presence of glucose fermentation. The Simmons Citrate showed the bacterium cannot solely use citrate as source of carbon.

What is the last test performed on E. coli?

The last test performed was the Urea test. The negative results explain that the bacterium did not have the enzyme urease, which breaks down urea. The bacterium did not produce an alkaline pH, but an acidic one. All of the test results came out correct, matching all the characteristic of E. coli (3).

What is the general microbiology course?

The General Microbiology I course taught students about many kinds of bacteria. Multiple procedures were practiced in the Microbiology lab to help students better understand how bacteria thrive, grow, and regenerate. These methods were used to determine which two unknown bacteria were present in the test tube.

Why is microbiology important in healthcare?

The way bacteria function and flourish is important, especially for people entering the Healthcare field, because it allows people to have a better understanding on how to work with or fight the bacteria. The General Microbiology I course taught students about many kinds of bacteria. Multiple procedures were practiced in the Microbiology lab to help students better understand how bacteria thrive, grow, and regenerate. These methods were used to determine which two unknown bacteria were present in the test tube.

How to make Gram positive bacteria colorless?

is done through several steps, first by adding crystal violet then when the iodine is added it. creates crystals which get stuck in the cell wall of Gram-positive bacteria. Ethanol is added to. get rid of the outer membrane of Gram-negative bacteria (which makes them colorless at this.

What enzymes are present in Pseudomonas?

This is probably due to certain enzymes (lipase, lecithinase, extracellular. protease) present with the genus. Pseudomonas, with its large genetic capacity, can produce a lot. of different enzymes that help it find several niches in the environment for it to utilize the.

Is an agar plate positive or negative?

Agar plate, which contains “an emulsion of DNA, peptides as a nutrient source, and methyl. green dye.”. The organism is positive when, after incubating for a given time (in this case, 24. hours), the organism should have a clearing around it. Conversely, a negative result would be no.

Why is it important to identify unknown bacteria?

This is important in the medical field because identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease. Also knowledge of different bacteria helped others make antibiotics used today. This lab was completed by using the methods learned thus far in identification of bacteria.

What is Kelsey Nolte's definition of microbiology?

Kelsey Nolte Microbiology Otero Junior College Mr. McClure Fall Semester 2014 Unknown Report Introduction The definition of Microbiology is the branch of science that deals with microorganisms. Microorganisms are very small organisms that require the use of a micro scope to visualize them, which are bacterium, viruses, fungi (the most common) and others which are not so common such as parasites. Microorganisms are an essential element to the field of microbiology because it allows researchers in

What is Lab12 in microbiology?

Lab12-Medical Microbiology- Part1– Differential Media/Biochemical Tests, Sp2012 (Set all of your margins to ½”) Purpose: The purpose of this lab is to help you become a little familiar with some of the tests that can be typically performed in a clinical or research lab facility. These tests may help in determining a particular pathogen’s growth needs. There are several sections to this lab. Find each section and complete the “Preparing for Class” sections. Preparing for class - Day 1 Read

What is the purpose of the lab?

The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways , each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests