unknown microbe from patient sample lab report

particular unknown microbe was isolated and characterized through physiological and genetic tests. The particular unknown microbe was determined to be a Staphylococcus epidermidis.

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What is an example of a lab report in microbiology?

Example of Unknown Lab Report, Microbiology. The quadrant streak helped spread out the bacteria and made it easier for the two different bacteria to make colonies that could be differentiated by the naked eye. This nutrient agar was incubated at 37° C for two days. The results were checked to see if two separate bacteria were growing.

How can we determine an unknown microbial species?

Determining an unknown microbial species using several different experimental techniques. Content may be subject to copyright. Our class completed several tests over approximately a two week period. These tests (among the ones we could possibly have) and by using a confirmatory test.

What procedures were used to find the two unknown bacteria?

The procedures from the laboratory manual by McDonald were used to accomplish the tests needed to find the two unknown bacteria (3). First, the bacteria mix inside the Unknown 109 tube was streaked across a nutrient agar plate using the quadrant streak technique.

Why is it important to identify the bacteria in medical records?

These bacteria must be able to be identified in order to treat patients properly, efficiently and safely. The unknown number 123 handed out by the Professor on March 20, 2014 contained both a gram positive bacteria and a gram negative bacteria.

How do you write an unknown bacteria for a lab report?

For example, instead of writing "I used a TSA agar plate to isolate my unknown," it is customary to write, "A trypticase soy agar (TSA) plate was used to isolate the unknown." It is also customary to write in the past tense for most of the report.

How can an unknown sample of bacteria be identified?

Identification of unknown bacteria is an integral module in most introductory Microbiology laboratory courses. This laboratory activity typically involves identifying bacteria based on Gram staining for morphology and gram reaction followed by studying their biochemical characteristics.

What are 3 tests we could use to identify an unknown bacteria?

The presence of catalase, gelatinase, oxidase, urease, for example, can be used to identify the species of bacteria. Biochemical reactions used in biochemical tests depend on the presence of such bacteria.

What is the purpose of unknown lab report?

The goal of this lab report is 1) to demonstrate comprehension of the methods and lab techniques learned during the semester 2) to explain the tests performed on each isolated unknown that led to the identification of each unknown 3) and to give a background on the characteristics, pathogenicity and some uses of one of ...

How do you identify a microbe?

PCR, including Real-Time PCR, is probably the most widely used molecular technique for identifying microbes. Using PCR, one can rapidly detect and identify microbial species directly from clinical samples, thus speeding up diagnostic procedures.

How do you identify bacteria in the lab?

Bacteria are identified routinely by morphological and biochemical tests, supplemented as needed by specialized tests such as serotyping and antibiotic inhibition patterns. Newer molecular techniques permit species to be identified by their genetic sequences, sometimes directly from the clinical specimen.

How do you do unknown in microbiology?

0:0431:58Unknown Project Beginning - YouTubeYouTubeStart of suggested clipEnd of suggested clipSo just to give you an idea of how the unknown project would work is that you would be given a brothMoreSo just to give you an idea of how the unknown project would work is that you would be given a broth. And in the broth that would contain two types of bacteria. You would have one gram positive.

What is the best method to identify a microorganism causing infection in a patient?

A sensitive test accurately screened blood samples for the presence of any of the more than 300 bacteria known to cause disease in people. If validated in larger studies, the method could be used to diagnose bacterial infections for precise treatment with antibiotics.

Why is it important to identify unknown microbes?

Accurate and definitive microorganism identification, including bacterial identification and pathogen detection, is essential for correct disease diagnosis, treatment of infection and trace-back of disease outbreaks associated with microbial infections.

What is the first step when given an unknown bacterial sample?

Pathogenic strains of bacteria tend to grow faster than non-pathogenic strains at 37°C, so researchers may set incubators at 25°C to restrict its growth. When given an unknown bacterial sample the first step is to expand the current bacterial population.

How do you write a microbiology lab report?

A lab report differs from a paper in that it has defined sections. The sections required vary from laboratory to laboratory but the standard outline for most lab reports in the biological science include: title, your name, purpose of the experiment, methods, results, discussion and conclusion, references.

What test was used to test for Gram Negative Unknown?

Maltose. Oxidase. An MSA and EMB test were performed using the Unknown 109 to try to promote the gram negative unknown to grow, but it did not work. The professor gave an Alternate #4 tube, so the gram negative unknown could be found.

What is the correct identification for gram positive bacteria?

The correct identification for the gram positive bacterium was Bacillus cereus. The B. cereus was found using the solution in the Unknown 109 test tube. Originally, a sample from the Unknown tube was supposed to be inoculated on a nutrient agar plate using the quadrant streak technique. This was to allow the gram positive and gram negative to be spread out, creating separate colonies. This never occurred. Several nutrient agar plates where inoculated and two different bacteria never grew. Then, it was concluded that only one bacterium was growing on the nutrient agar, so a gram stain was performed. A gram positive rod was the outcome, ruling out the gram positive cocci. The Methyl Red test concluded positive for the presence of glucose fermenters. The Casein test concluded negative for the bacterium having casease to hydrolyze casein. This was an incorrect finding because B. cereus does have casease. The finding could have been incorrect because the clearing around the bacterium might not have been as clear for the tester to notice. The Glycerol and Maltose test showed fermentation for carbohydrates by changing the solution from yellow to red. The Maltose test showed positive, but the Glycerol test show incorrect results for negative. The two tests call for the bacterium to be in a liquid form when added to the test tubes, but the test was done from the bacterium being on an agar jell. The bacterium might not have mixed well with the Glycerol solution, creating an improper answer. Lastly, an Oxidase test showed positive for the bacterium having cytochrome c. This completed the search for the Gram positive rod: B. cereus (3).

Why is the casein test negative?

The Casein test concluded negative for the bacterium having casease to hydrolyze casein. This was an incorrect finding because B. cereus does have casease. The finding could have been incorrect because the clearing around the bacterium might not have been as clear for the tester to notice.

What is the significance of the EMB test?

They were positive and acidic. The EMB test produced green metallic colonies, which was a big flag that the bacterium might be Escherichia coli. The Methyl Red test was positive for the presence of glucose fermentation. The Simmons Citrate showed the bacterium cannot solely use citrate as source of carbon.

What is the last test performed on E. coli?

The last test performed was the Urea test. The negative results explain that the bacterium did not have the enzyme urease, which breaks down urea. The bacterium did not produce an alkaline pH, but an acidic one. All of the test results came out correct, matching all the characteristic of E. coli (3).

What is the general microbiology course?

The General Microbiology I course taught students about many kinds of bacteria. Multiple procedures were practiced in the Microbiology lab to help students better understand how bacteria thrive, grow, and regenerate. These methods were used to determine which two unknown bacteria were present in the test tube.

Why is microbiology important in healthcare?

The way bacteria function and flourish is important, especially for people entering the Healthcare field, because it allows people to have a better understanding on how to work with or fight the bacteria. The General Microbiology I course taught students about many kinds of bacteria. Multiple procedures were practiced in the Microbiology lab to help students better understand how bacteria thrive, grow, and regenerate. These methods were used to determine which two unknown bacteria were present in the test tube.

How to make Gram positive bacteria colorless?

is done through several steps, first by adding crystal violet then when the iodine is added it. creates crystals which get stuck in the cell wall of Gram-positive bacteria. Ethanol is added to. get rid of the outer membrane of Gram-negative bacteria (which makes them colorless at this.

What enzymes are present in Pseudomonas?

This is probably due to certain enzymes (lipase, lecithinase, extracellular. protease) present with the genus. Pseudomonas, with its large genetic capacity, can produce a lot. of different enzymes that help it find several niches in the environment for it to utilize the.

Is an agar plate positive or negative?

Agar plate, which contains “an emulsion of DNA, peptides as a nutrient source, and methyl. green dye.”. The organism is positive when, after incubating for a given time (in this case, 24. hours), the organism should have a clearing around it. Conversely, a negative result would be no.

How to identify unknown bacteria?

The first method used to identify the unknown bacteria was an isolation streak plate, which utilized four streaks of the unknown mixture onto a nutrient agar plate, via inoculating loop. This procedure was used in order to attempt to isolate separate pure colonies from the unknown mixture.

What is the bacterium test for Gram negative bacteria?

The final test completed on the Gram negative bacterium was a Lactose test, specifically , EMB, also known as an Eosin Methylene Blue agar plate. For this test, the isolated Gram negative bacterium was streak inoculated onto the agar plate and incubated.

Why is Bacillus subtilis used in genetic research?

Scientist use Bacillus subtilis in order to promote genetic research and due to its highly genetic manipulability levels, it is easier to conduct testing on (Swartzburg, 2009).

Why is mannitol salt agar used in a Gram positive test?

The purpose of this test was to isolate the Gram positive bacterium. This was possible because Mannitol Salt Agar is a selective medium (along with differential) that only allows for the growth of specific Gram positive bacteria due to its high salt content (McDonald et al., 2011).

What does it mean when a maltose test is negative?

Once the Maltose test was confirmed as negative, it indicated that Bacillus subtilis was the unknown bacterium in question. However, while a correct conclusion was derived through these two tests, problems were encountered. At the same time the maltose test was being completed, a mannitol test was run.

What test was used to test Gram positive?

Secondly for this specimen, a Simmon’s Citrate test was used. In order to complete this test, the isolated bacterium (Gram positive) was spread across the Simmon’s Citrate slant, in order to promote growth.

What was the first test performed to confirm a Gram positive?

Purple rods were observed under a light microscope, confirming this. The first test performed was a Simmon’s citrate, which resulted in a Positive reading.

What bacteria turned out to be Gram negative?

For further accurate results inoculated plate was kept in the incubator for another 48 hours. The result was confirmed to be Gram negative bacillus.

Why is knowing the differences in bacterial structure and response important?

Knowing the differences in bacterial structure and response is essential for all future and current healthcare providers. Dealing with people’s lives and health is it necessary to know what kind of antibiotic can be prescribed to a patient in order to fix his problem.

What is the Gram positive bacteria?

According to microbiology instructor Gram positive bacteria was Staphylococcus aureus, that leads to the conclusion that final Urea test had shown wrong results. For future healthcare providers, it is extremely important to be able to identify what kind of pathogen is thriving in a patient’s organism.

Why did the nitrate and MR-VP test turn out to be negative?

Nitrate and MR-VP tests were supposed to be redone, due to a technical mistake. After accurately conducted test, the results of Nitrate test and MR-VP tests turned out to be negative. Indole test was negative as well and didn’t show any color change.

Why do different microorganisms require different environments?

Different microorganisms require various environments in order to replicate and to become dangerous for a person’s health. An integral part of any medical treatment is to be able to recognize and identify a specific bacterium that can create potentially a big problem for a patient.

Why are microorganisms important?

Microorganisms cause a great amount of diseases. For healthcare providers it is very important to be aware of what organisms are pathogenic and cause a disease and, therefore, to find an appropriate treatment . Different microorganisms require various environments in order to replicate and to become dangerous for a person’s health.

Can staph be spread through physical contact?

Under normal circumstances it doesn’t cause any problems , however, if a patient has a compromised immune system Staphylococcus aureus can become a serious problem and lead to Methicillin resistant Staphylococcus aureus (MRSA). MRSA is a type of staph bacteria that can be easily spread through a physical contact with a sick person.